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KMID : 0378019700130010108
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New Medical Journal
1970 Volume.13 No. 1 p.108 ~ p.115
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Insulin Effect on Oxidative Metabolism of C^(14)-acetate in the Cardinal Organ Tissues of Rat
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Abstract
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Tissue homogenates of brain, liver, heart and kidney excised from normal and alloxan diabetic rats were incubated in the medium in which C14-acetate was added so as to maintain the concentration of 20 mg%. After 3 hours in incubation period, CO2 samples trapped by alkaline in the incubation fl4k were analyzed for their total COz production rates and radioactivities. Fractions of CO2 yields fron! medium acetate to total CO2 production rates were determined by relative specific activity (RSA) which is obtained with ratio between specific activities of CO2 and medium C14-acetate.
The results were as follows:
1. In the brain tissue, total CO2 production rates were 15.0±1.5 pM/hr/gm in control and 15. 4±1. 3 pM/hr/gm in alloxan diabetic group. RSA was averaged 1.07±0.10% in control and 0.98±0.16% in diabetic goup. Consquently, there are no difference in CO2 yield from medium C14-acetate between contol and diabetic group in brain tissues.
2. In the kidney tissue, total CO2 production rates, RSA and CO2 yield from acetate were 12.6±1.0 uM /hr/gm, 2.19±0.17% and 0. 27±0. 01 pM/hr/gm in control group and 8. 6±1. 5 I_M/hr/gm, 1. 19± 0. 24% and 0. 10±0. 04 pM/hr/gm respectively in the alloxan diabetic group.
3. In the cardiac tissue, mean values of total CO2 production rate in control and alloxan diabetic group were 9. 0±1. 5 and 8.6±1.5 ppM/hr/gm respectively. RSA were 1.50±0.17 and 1.59±0.13% in alloxan diabetic group. CO2 yield from acetate showed same values in both group.
4. In the liver tissue, total CO2 ptoduction rates showed almost same in control and alloxan diabetic group but RSA is increased about 34% in diabetic group. Accordingly, CO2 yield from acetate also increased in diabetic group.
From the above data, insulin defect did not effect on oxidative metabolism of acetate in the cardinal organ such as brain and heart but increased in acetate oxidation in liver tissue.
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